The Conner Lab recently published an article titled “Characteristics of tiger moth (Erebidae: Arctiinae) anti-bat sounds can be predicted from tymbal morphology.” In this article, the facilities Amray 1810 SEM microscope was used to image the thorax of tiger moths. Undergraduate researchers Erika Metzler, James Clemmons, Thisbe Scholfield-Johnson, and Kahla Seymour participated in obtaining the SEM…
At Wake Forest we pride we pride ourselves on the wonderful collaborative environment between departments. In the imaging field we move forward with collaboration between biologists, chemists, physicists, engineers, and even computer scientists. The Lukesh Lab has recently published two papers on fluorescent probes they have developed that allow biologists to visualize lipid droplets and…
This year the core has been quite busy, and many papers have been published! Check out all the work that has been done! J. K. Muhlemann, T. L. B. Younts, G. K. Muday, Flavonols control pollen tube growth and integrity by regulating ROS homeostasis during high-temperature stress. PNAS. 115, E11188–E11197 (2018). E.R. Shuppe, M.J. Fuxjager,…
The Johnson Lab recently published “Unexpected role of a conserved domain in the first extracellular loop in G protein-coupled receptor trafficking.” The lead author was graduate student Michael Rizzo who performed his experiments on the Zeiss 710 confocal microscope in the Winston Hall microscopy core. Also involved in this study were undergraduate researchers John “Jack”…
We keep statistics for all sorts of things from July 1- June 30th every year. One of the most important statistic for us is the number and type of publications that come from researchers utilizing our core facility. We are hoping with the second site Downtown, 3 more pieces of equipment, and new hires we…
Abstract: Gradients of the plant hormone auxin, which depend on its active intercellular transport, are crucial for the maintenance of root meristematic activity. This directional transport is largely orchestrated by a complex interaction of specific influx and efflux carriers that mediate the auxin flow into and out of cells, respectively. Besides these transport proteins, plant-specific polyphenolic compounds knownasflavonols have beenshownto act…
Plant root development is mediated by the concerted action of the auxin and cytokinin phytohormones, with cytokinin serving as an antagonist of auxin transport. Here, we identify the AUXIN UP-REGULATED F-BOX PROTEIN1 (AUF1) and its potential paralog AUF2 as important positive modifiers of root elongation that tether auxin movements to cytokinin signaling in Arabidopsis (Arabidopsis…
Auxin and ethylene are key regulators of plant growth and development, and thus the transcriptional networks that mediate responses to these hormones have been the subject of intense research. This study dissected the hormonal cross talk regulating the synthesis of flavonols and examined their impact on root growth and development. We analyzed the effects of…
Published estimates of the number of ovarioles found in the ovaries of honey bee, Apis mellifera L. (Hymenoptera: Apidae) queens range from 100 to 180 per ovary. Within the context of a large-scale study designed to assay the overall quality of queens obtained from various commercial sources, a simple histology-based method for accurate determination of ovariole number…
We used genetic and molecular approaches to identify mechanisms by which the gaseous plant hormone ethylene reduces lateral root formation and enhances polar transport of the hormone auxin. Arabidopsis thaliana mutants, aux1, lax3, pin3 and pin7, which are defective in auxin influx and efflux proteins, were less sensitive to the inhibition of lateral root formation…
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