Wake Forest University Biology, Wake Downtown, and the Center for Molecular signaling provide financial support for the service contracts. In order to demonstrate how our core supports the research community, we ask that you acknowledge the core in your publications. Please edit as you see fit—
- ‘the authors gratefully acknowledge the Wake Forest University Microscopic Imaging Core (RRID:SCR_02975) for their support & assistance in this work’
- ‘the authors thank *** of Wake Forest University Microscopic Imaging Core (RRID:SCR_02975) for their support & assistance in this work’
Want to know more about our guidelines? See our adapted RMS acknowledgment PDF
Materials & Methods
Please provide at least this information for your materials and methods. See individual instrument pages for instrument names, objectives, and laser lines. This information can also be found in the metadata of your raw image files. Example from Harvard Nikon Imaging Center-
All images were collected with a [INSERT CONFOCAL MODEL if appliciable] on a [Zeiss AxioObserver Z1, AxioZoom V16, etc) microscope equipped with [INSERT OBJECTIVE LENS Correction Mag/NA]. [INSERT FLUOROPHORE – Be specific!! If you used a fluorescent protein, state the exact variant – e.g., mEGFP not GFP, or TagRFP2 not RFP] fluorescence was excited with the [INSERT LASER LINE] line from a [INSERT LASER POWER] mW [INSERT LASER TYPE] (selected with an AOTF) and collected with a [INSERT DICHROIC MIRROR MODEL] (Chroma) and a [INSERT EMISSION FILTER Peak wavelength/bandwidth]. Images were acquired with a [INSERT CAMERA MODEL if not confocal] controlled with [INSERT ACQUISITION SOFTWARE] software. Z-series optical sections were collected with a step-size of X microns, using the [MODEL FOCUS MOTOR]. Z-series are displayed as maximum z-projections, and gamma, brightness, and contrast were adjusted (identically for compared image sets) using [IMAGE ANALYSIS SOFTWARE] software [CITATION FOR OPEN SOURCE SOFTWARE].
Additions for live cell imaging
Cells were grown on No. 1.5 coverslips and mounted in a [INSERT MODEL AND TYPE (stage top or enclosure) OF HEATED CHAMBER] heated chamber warmed to X°C. [INSERT TISSUE CULTURE MEDIA and METHOD OF BUFFERING] and without phenol red was used during image acquisition.
For time-lapse experiments, images were collected every X min, using an exposure time of X. At each time-point, X z-series optical sections were collected with a step-size of X microns, using the [MODEL FOCUS MOTOR]. Multiple stage positions were collected using a [MODEL MOTORIZED STAGE].