Since I joined in May we have put a lot of work into updating the website. There are two new areas on the website that I invite you to check out. We are not the only microscope core on campus. Under instruments we have Microscopes Across Campus. This gives you links to cores/departments across Wake that…
Justin Watkins of the Muday lab and long time microscopy core user successfully defended his dissertation on 8/3! Justin received his B.S. in Biological Sciences & Plant Biology from N.C. State before joining the Wake Forest Biology department as a graduate student. Justin’s next stop will be Utah to do postdoctoral research in…
This week James Privitt defended his thesis and passed! If you see him, please congratulate him. James did his thesis work under Dr. Susan Fahrbach (lab page) and was an extensive user of the LSM 710 on the Reynolda campus. In the fall he will be starting a PhD program at Cornell. The following…
In January, we added the LSM 880 confocal microscope to our facility. Everyone has loved the extra sensitivity and the crisp images that they have been getting from the new system. There is a feature that many don’t know about, though. The Airyscan detector! A new detector, how could that be exciting? The Airyscan is…
Abstract: Gradients of the plant hormone auxin, which depend on its active intercellular transport, are crucial for the maintenance of root meristematic activity. This directional transport is largely orchestrated by a complex interaction of specific influx and efflux carriers that mediate the auxin flow into and out of cells, respectively. Besides these transport proteins, plant-specific polyphenolic compounds knownasflavonols have beenshownto act…
Accessories have been purchased or upgraded to create an inverted system capable of performing rapid ratio imaging experiments. A dual channel perfusion heater, solution heater, and 35mm chamber with stage insert were purchased from Warner Instruments. A 6-position excitation filter wheel, powered by a new Mac6000 controller from Ludl Electronic Products, will allow 30msec switching…
For those of you interested in performing spectral separation using the LSM 710, check out the protocol recently uploaded to this site. This protocol, developed by Dan Lewis of the Muday lab, outlines how to acquire and unmix samples containing overlapping fluorescent spectra. The protocol can be found under the Resources section of this website…
The Microscopy Facility has recently added an EC Plan-Neofluar 1.25x objective to the Zeiss Axioplan upright microscope. This objective has a numerical aperture of 0.03 and a working distance of 3.9 mm. This non-immersion objective is designed for 0.17 coverslip thickness and provides a 25mm field of view. EC Plan-Neofluar’s are excellent universal objectives that…
On November 9th, 2011, the WFU Biology, Chemistry, and Physics departments, the WFU Center for Molecular Communication and Signaling, and Carl Zeiss Microscopy hosted a workshop on fluorescent microscopy. The workshop was outstanding and the turnout was amazing. 82 scientists from institutions across North Carolina attended lectures on fluorescent proteins and probes for live cell…
Plant root development is mediated by the concerted action of the auxin and cytokinin phytohormones, with cytokinin serving as an antagonist of auxin transport. Here, we identify the AUXIN UP-REGULATED F-BOX PROTEIN1 (AUF1) and its potential paralog AUF2 as important positive modifiers of root elongation that tether auxin movements to cytokinin signaling in Arabidopsis (Arabidopsis…
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