New 1.25x Objective for the Upright Microscope

The Microscopy Facility has recently added an EC Plan-Neofluar 1.25x objective to the Zeiss Axioplan upright microscope.  This objective has a numerical aperture of 0.03 and a working distance of 3.9 mm.  This non-immersion objective is designed for 0.17 coverslip thickness and provides a 25mm field of view.  EC Plan-Neofluar’s are excellent universal objectives that provide strong performance in field flatness, color correction, and light transmission from ~400nm to the IR range.

Application Note: In order to capture the entire field of view, it is necessary to remove the condenser lens.  Please contact the Facility Director to learn how to do this.