Wake Forest Biology Department
The facility has acquired a Tamron XR long working distance macro lens for use with the Nikon D50 camera. When paired with the reflected light illumination table and camera stand in Winston Hall 002, this system provides an excellent method for acquiring images for field sizes ranging from 2-15 cm. This system provides a high…
Tile scanning mode for the Zeiss 710 confocal now includes the capability to assign an overlap percentage for seamless stitching of tiled images. This feature corrects slight offset and misalignment that formerly could be detected between individual tile scanned fields.
A new, more powerful workstation has been added to the Zeiss 710 confocal system. This 64-bit, Windows 7 system offers faster processing speed and 16GB of RAM. Zen software version 2012 is now in use on this CPU.
A 50 mW handheld blue laser (447 nm) is now located in the confocal suite. This device excites green fluorescent proteins such as GFP with fluorescent emission of 480-750 nm observed using the Thor Labs LG2 protective glasses. Use the handheld laser to genotype your transgenic organism by eye before dissection/mounting for confocal observation, or use it as…
Accessories have been purchased or upgraded to create an inverted system capable of performing rapid ratio imaging experiments. A dual channel perfusion heater, solution heater, and 35mm chamber with stage insert were purchased from Warner Instruments. A 6-position excitation filter wheel, powered by a new Mac6000 controller from Ludl Electronic Products, will allow 30msec switching…
For those of you interested in performing spectral separation using the LSM 710, check out the protocol recently uploaded to this site. This protocol, developed by Dan Lewis of the Muday lab, outlines how to acquire and unmix samples containing overlapping fluorescent spectra. The protocol can be found under the Resources section of this website…
The Microscopy Facility has recently added an EC Plan-Neofluar 1.25x objective to the Zeiss Axioplan upright microscope. This objective has a numerical aperture of 0.03 and a working distance of 3.9 mm. This non-immersion objective is designed for 0.17 coverslip thickness and provides a 25mm field of view. EC Plan-Neofluar’s are excellent universal objectives that…
On November 9th, 2011, the WFU Biology, Chemistry, and Physics departments, the WFU Center for Molecular Communication and Signaling, and Carl Zeiss Microscopy hosted a workshop on fluorescent microscopy. The workshop was outstanding and the turnout was amazing. 82 scientists from institutions across North Carolina attended lectures on fluorescent proteins and probes for live cell…
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