Welcome to Dr. Heather Brown-Harding, our new microscopy manager stationed in the Wake Downtown Microscopy facility. Heather received her Ph.D. in Microbiology and Immunology from the University of Florida in 2013. Before joining us, she served as a Key Account Manager for Zeiss Microscopy in the greater Boston area. With lots of energy, extensive microscopy…
An entirely new microscopy facility is now open in room 2806 of Wake Downtown. This facility features an all new Zeiss 880 confocal with Airyscan, and a V-16 macroview microscope. Drop by to tour the equipment and discuss possible experimental and teaching applications, or make an appointment with the director for equipment training.
New student microscopes now available for Biology upper division teaching exercises. Zeiss Primostar compound microscopes include phase contrast and color digital cameras. Three Primostar microscopes feature push button LED green channel fluorescence, courtesy of the Biology Neuroscience Program. An additional Primostar microscope is available without fluorescence, but featuring a wider variety of phase contrast objectives.
The Compresstome tissue sectioning device offers an alternative to vibratome slicing, and features superior tissue block support and sectioning in the range of 30-500um thickness. This technique is suitable for live or fixed, non-frozen tissues. .
Sabrina Setaro and colleagues have created a Citizen Sort game that is now open to the public. After collecting a series of confocal image stacks of fluorescently stained fungi on various plant roots, this group seeks to utilize the power of citizen science to analyze and validate this large volume of imaging data. Test your…
Imaris software is now in use that allows advanced 3-D reconstruction, viewing, and presentation of image stacks. Rendered images can be visualized and manipulated in standard mode, or as surface/contour plots. A host of analytic segmentation and measurement tools are also available.
Hamamatsu HCImage capture and analysis software has been added for the Zeiss AxioObserver inverted fluorescent microscope. HCImage includes real-time image analysis features, an intuitive control module for acquisition settings, and advanced analysis paradigms such as ratiometric calculation and display. This software is intended as a replacement for the Volocity acquisition system.
The confocal microscope has been upgraded with a new 10X 0.45 NA Plan Apo lens. This lens will substantially improve image quality for lower magnification acquisition.
A high quality stereoscope has been relocated to the confocal suite. This stereoscope can be used as a teaching microscope with the capability for live sample digital viewing via the associated Moticam connection to a nearby monitor. A NightSea fluorescence adapter system was also added to this system that allows for LED fluorescent visualization of…
The facility has acquired a Tamron XR long working distance macro lens for use with the Nikon D50 camera. When paired with the reflected light illumination table and camera stand in Winston Hall 002, this system provides an excellent method for acquiring images for field sizes ranging from 2-15 cm. This system provides a high…
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