Accessories have been purchased or upgraded to create an inverted system capable of performing rapid ratio imaging experiments.  A dual channel perfusion heater, solution heater, and 35mm chamber with stage insert were purchased from Warner Instruments. A 6-position excitation filter wheel, powered by a new Mac6000 controller from Ludl Electronic Products, will allow 30msec switching of excitation wavelengths.  The ET-FURA2 cube, by Chroma, will allow alternating 340 and 380 excitation with a 470-550nm emission.  The microscope and accessories will be driven by Volocity 6.1.