Abstract: Gradients of the plant hormone auxin, which depend on its active intercellular transport, are crucial for the maintenance of root meristematic activity. This directional transport is largely orchestrated by a complex interaction of specific influx and efflux carriers that mediate the auxin flow into and…
read moreAccessories have been purchased or upgraded to create an inverted system capable of performing rapid ratio imaging experiments. A dual channel perfusion heater, solution heater, and 35mm chamber with stage insert were purchased from Warner Instruments. A 6-position excitation filter wheel, powered…
read moreFor those of you interested in performing spectral separation using the LSM 710, check out the protocol recently uploaded to this site. This protocol, developed by Dan Lewis of the Muday lab, outlines how to acquire and unmix samples containing overlapping fluorescent…
read moreThe Microscopy Facility has recently added an EC Plan-Neofluar 1.25x objective to the Zeiss Axioplan upright microscope. This objective has a numerical aperture of 0.03 and a working distance of 3.9 mm. This non-immersion objective is designed for 0.17 coverslip thickness and…
read moreOn November 9th, 2011, the WFU Biology, Chemistry, and Physics departments, the WFU Center for Molecular Communication and Signaling, and Carl Zeiss Microscopy hosted a workshop on fluorescent microscopy. The workshop was outstanding and the turnout was amazing. 82 scientists from institutions…
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Plant root development is mediated by the concerted action of the auxin and cytokinin phytohormones, with cytokinin serving as an antagonist of auxin transport. Here, we identify the AUXIN UP-REGULATED F-BOX PROTEIN1 (AUF1) and its potential paralog AUF2 as important positive modifiers…
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Auxin and ethylene are key regulators of plant growth and development, and thus the transcriptional networks that mediate responses to these hormones have been the subject of intense research. This study dissected the hormonal cross talk regulating the synthesis of flavonols and…
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Published estimates of the number of ovarioles found in the ovaries of honey bee, Apis mellifera L. (Hymenoptera: Apidae) queens range from 100 to 180 per ovary. Within the context of a large-scale study designed to assay the overall quality of queens obtained from…
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We used genetic and molecular approaches to identify mechanisms by which the gaseous plant hormone ethylene reduces lateral root formation and enhances polar transport of the hormone auxin. Arabidopsis thaliana mutants, aux1, lax3, pin3 and pin7, which are defective in auxin influx…
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Human mismatch repair proteins MSH2-MSH6 play an essential role in maintaining genetic stability and preventing disease. While protein functions have been extensively studied, the substantial amino-terminal region (NTR*) of MSH6 that is unique to eukaryotic proteins, has mostly evaded functional characterization. We…
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